TRIP - Transgenic Rats ImmunoPhenomic

Scientific head

Technical participants

Laurent Tesson
Research Assistant
Laure-Hélène Ouisse
Research Assistant
Vanessa Quillaud-Chenouard
Research Assistant

TRIP platform has been created in 1996 and has ever since developed Knock-Out (KO) or Knock-In (KI) rat research models for all (academic or private labs, French or international companies…)

  • Gene or large genomic locus function analysis: Gene(s) KO
  • SNP studies: KI to induce mutation of interest
  • Cellular or protein tracking: reporter KI in frame with your marker protein
  • Protein or reporter gene overexpression: KI of your protein of interest or reporter under the control of ubiquitous promoter
  • Spatio-temporal control by conditional models
  • Human cells or tissues engraftment: immunodeficient models

To discuss your own project or if you are interested in our available research models, please contact Ignacio Anegon

For a review on rat genome editing and rat models of immune genes and genetic diseases see Chenouard V, Remy S, Tesson L, Ménoret S, Ouisse LH, Cherifi Y, Anegon I. Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models. Front Genet. 2021 Apr 20;12:615491. PMID: 33959146

 

AVAILABLE RESEARCH MODELS

Reporter GFP rats (alive & cryoconserved)

Remy, S. et al. (2010). New lines of GFP transgenic rats relevant for regenerative medicine and gene therapy. Transgenic Research19(5), 745‑763. https://doi.org/10.1007/s11248-009-9352-2

 

Immunodeficient rats

  • RRG KO (rIL2Rg KO / rRag-1 KO) (cryoconserved)Ménoret, S., et al. (2018). Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation102(8), 1271‑1278. https://doi.org/10.1097/TP.0000000000002251
  • RRGS (RRG KO with human SIRPα) (alive & cryoconserved)Ménoret, S., et al. 2019. « In Vivo Analysis of Human Immune Responses in Immunodeficient Rats ». Transplantation, novembre. https://doi.org/10.1097/TP.0000000000003047

 

Duchenne Muscular Dystrophy  (DMD) disease rat model (alive & cryoconserved)

Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., … Huchet, C. (2014). Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PloS One9(10), e110371. https://doi.org/10.1371/journal.pone.0110371

Ouisse, Laure-Hélène, Séverine Remy, Aude Lafoux, Thibaut Larcher, Laurent Tesson, Vanessa Chenouard, Carole Guillonneau, et al. 2019. « Immunophenotype of a Rat Model of Duchenne’s Disease and Demonstration of Improved Muscle Strength After Anti-CD45RC Antibody Treatment ». Frontiers in Immunology 10 (septembre): 2131. https://doi.org/10.3389/fimmu.2019.02131

Caudal, Dorian, Virginie François, Aude Lafoux, Mireille Ledevin, Ignacio Anegon, Caroline Le Guiner, Thibaut Larcher, et Corinne Huchet. 2020. « Characterization of brain dystrophins absence and impact in dystrophin-deficient Dmdmdx rat model ». PLoS ONE 15 (3). https://doi.org/10.1371/journal.pone.0230083

 

Other reporter and conditional models are available in repositories and vendors

 

PARTNERS

 

PUBLICATIONS

2020

  • Menchaca, A., Dos Santos-Neto, P. C., Souza-Neves, M., Cuadro, F., Mulet, A. P., Tesson, L., Chenouard, V., Guiffès, A., Heslan, J. M., Gantier, M., Anegón, I., & Crispo, M. (2020). Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair. Scientific Reports, 10(1), 5995. https://doi.org/10.1038/s41598-020-62879-y
  • Ménoret, S., Ouisse, L.-H., Tesson, L., Remy, S., Usal, C., Guiffes, A., Chenouard, V., Royer, P.-J., Evanno, G., Vanhove, B., Piaggio, E., & Anegon, I. (2020). In Vivo Analysis of Human Immune Responses in Immunodeficient Rats. Transplantation, 104(4), 715–723. https://doi.org/10.1097/TP.0000000000003047
  • Manaud, G., Nossent, E. J., Lambert, M., Ghigna, M.-R., Boët, A., Vinhas, M.-C., Ranchoux, B., Dumas, S. J., Courboulin, A., Girerd, B., Soubrier, F., Bignard, J., Claude, O., Lecerf, F., Hautefort, A., Florio, M., Sun, B., Nadaud, S., Verleden, S. E., … Perros, F. (2020). Comparison of Human and Experimental Pulmonary Veno-Occlusive Disease. American Journal of Respiratory Cell and Molecular Biology. https://doi.org/10.1165/rcmb.2019-0015OC
  • Caudal, D., François, V., Lafoux, A., Ledevin, M., Anegon, I., Le Guiner, C., Larcher, T., & Huchet, C. (2020). Characterization of brain dystrophins absence and impact in dystrophin-deficient Dmdmdx rat model. PLoS ONE, 15(3). https://doi.org/10.1371/journal.pone.0230083
  • Adigbli, G., Ménoret, S., Cross, A. R., Hester, J., Issa, F., & Anegon, I. (2020). Humanization of Immunodeficient Animals for the Modeling of Transplantation, Graft Versus Host Disease and Regenerative Medicine. Transplantation. https://doi.org/10.1097/TP.0000000000003177
  • Fourrier, A., Delbos, F., Menoret, S., Collet, C., Thi Thuy, L. T., Myara, A., Petit, F., Tolosa, L., Laplanche, S., Gómez-Lechón, M. J., Labrune, P., Anegon, I., Vallier, L., Garnier, D., & Nguyen, T. H. (2020). Regenerative cell therapy for the treatment of hyperbilirubinemic Gunn rats with fresh and frozen human induced pluripotent stem cells-derived hepatic stem cells. Xenotransplantation, 27(1), e12544. https://doi.org/10.1111/xen.12544

2019

  • Kenawi, Moussa, Emmanuel Rouger, Marie-Laure Island, Patricia Leroyer, François Robin, Séverine Rémy, Laurent Tesson, et al. (2019). Ceruloplasmin Deficiency Does Not Induce Macrophagic Iron Overload: Lessons from a New Rat Model of Hereditary Aceruloplasminemia. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, septembre, fj201901106R. https://doi.org/10.1096/fj.201901106R
  • Lambert, Mélanie, Véronique Capuano, Angèle Boet, Laurent Tesson, Thomas Bertero, Morad K. Nakhleh, Séverine Remy, et al. (2019). Characterization of Kcnk3-Mutated Rat, a Novel Model of Pulmonary Hypertension. Circulation Research 125 (7): 678‑95. https://doi.org/10.1161/CIRCRESAHA.119.314793
  • Ouisse, Laure-Hélène, Séverine Remy, Aude Lafoux, Thibaut Larcher, Laurent Tesson, Vanessa Chenouard, Carole Guillonneau, et al. (2019). Immunophenotype of a Rat Model of Duchenne’s Disease and Demonstration of Improved Muscle Strength After Anti-CD45RC Antibody Treatment. Frontiers in Immunology 10: 2131. https://doi.org/10.3389/fimmu.2019.02131

2018

  • Clarke, Starlynn C., Biao Ma, Nathan D. Trinklein, Ute Schellenberger, Michael J. Osborn, Laure-Hélène Ouisse, Andrew Boudreau, et al. (2018). Multispecific Antibody Development Platform Based on Human Heavy Chain Antibodies. Frontiers in Immunology 9: 3037. https://doi.org/10.3389/fimmu.2018.03037
  • Charpentier, M., Khedher, A. H. Y., Menoret, S., Brion, A., Lamribet, K., Dardillac, E., … Concordet, J. P. (2018). CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nature Communications, 9(1), 1133. https://doi.org/10.1038/s41467-018-03475-7
  • Ménoret, S., Ouisse, L.-H., Tesson, L., Delbos, F., Garnier, D., Remy, S., … Anegon, I. (2018). Generation of Immunodeficient Rats With Rag1 and Il2rg Gene Deletions and Human Tissue Grafting Models. Transplantation, 102(8), 1271‑1278. https://doi.org/10.1097/TP.0000000000002251
  • Ossart, J., Moreau, A., Autrusseau, E., Ménoret, S., Martin, J. C., Besnard, M., … Guillonneau, C. (2018). Breakdown of Immune Tolerance in AIRE-Deficient Rats Induces a Severe Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy-like Autoimmune Disease. Journal of Immunology (Baltimore, Md.: 1950), 201(3), 874‑887. https://doi.org/10.4049/jimmunol.1701318
  • Ouisse, L.-H., Remy, S., Lafoux, A., Larcher, T., Tesson, L., Chenouard, V., … Anegon, I. (2018). Immunological characterization of a rat model of Duchenne’s disease and demonstration of improved muscle strength after anti-CD45RC antibody treatment. BioRxiv, 407023. https://doi.org/10.1101/407023

2017

  • Lopez Robles, M. D., Pallier, A., Huchet, V., Le Texier, L., Remy, S., Braudeau, C., … Chiffoleau, E. (2017). Cell-surface C-type lectin-like receptor CLEC-1 dampens dendritic cell activation and downstream Th17 responses. Blood Advances, 1(9), 557‑568. https://doi.org/10.1182/bloodadvances.2016002360
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Ouisse, L.-H., Brusselle, L., … Anegon, I. (2017). Advances in transgenic animal models and techniques. Transgenic Research, 26(5), 703‑708. https://doi.org/10.1007/s11248-017-0038-x
  • Ouisse, L.-H., Gautreau-Rolland, L., Devilder, M.-C., Osborn, M., Moyon, M., Visentin, J., … Saulquin, X. (2017). Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnology, 17(1), 3. https://doi.org/10.1186/s12896-016-0322-5
  • Remy, S., Chenouard, V., Tesson, L., Usal, C., Ménoret, S., Brusselle, L., … Anegon, I. (2017). Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation. Scientific Reports, 7(1), 16554. https://doi.org/10.1038/s41598-017-16328-y

2016

  • Chenouard, V., Brusselle, L., Heslan, J.-M., Remy, S., Ménoret, S., Usal, C., … Tesson, L. (2016). A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals: A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 43(5), 341‑348. https://doi.org/10.1016/j.jgg.2016.04.005
  • Fontaniere, S., Braun V, Ménoret S, Anegon I, Merrouche C, Fraichard A, & Cherifi Y. (2016). Pronuclear injection for the production of transgenic rats. Cold Spring Harbor protocols.
  • Jung, C. J., Ménoret, S., Brusselle, L., Tesson, L., Usal, C., Chenouard, V., … Anegon, I. (2016). Comparative Analysis of piggyBac, CRISPR/Cas9 and TALEN Mediated BAC Transgenesis in the Zygote for the Generation of Humanized SIRPA Rats. Scientific Reports, 6, 31455. https://doi.org/10.1038/srep31455
  • Martin, J. C., Bériou, G., Heslan, M., Bossard, C., Jarry, A., Abidi, A., … Josien, R. (2016). IL-22BP is produced by eosinophils in human gut and blocks IL-22 protective actions during colitis. Mucosal Immunology, 9(2), 539‑549. https://doi.org/10.1038/mi.2015.83
  • Menchaca, A., Anegon, I., Whitelaw, C. B. A., Baldassarre, H., & Crispo, M. (2016). New insights and current tools for genetically engineered (GE) sheep and goats. Theriogenology, 86(1), 160‑169. https://doi.org/10.1016/j.theriogenology.2016.04.028
  • Renaud, J.-B., Boix, C., Charpentier, M., De Cian, A., Cochennec, J., Duvernois-Berthet, E., … Giovannangeli, C. (2016). Improved Genome Editing Efficiency and Flexibility Using Modified Oligonucleotides with TALEN and CRISPR-Cas9 Nucleases. Cell Reports, 14(9), 2263‑2272. https://doi.org/10.1016/j.celrep.2016.02.018
  • Tesson, L., Remy, S., Ménoret, S., Usal, C., Thinard, R., Savignard, C., … Anegon, I. (2016). Genome Editing in Rats Using TALE Nucleases. Methods in Molecular Biology (Clifton, N.J.), 1338, 245‑259. https://doi.org/10.1007/978-1-4939-2932-0_18

2015

  • Crispo, M., Mulet, A. P., Tesson, L., Barrera, N., Cuadro, F., dos Santos-Neto, P. C., … Menchaca, A. (2015). Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes. PloS One, 10(8), e0136690. https://doi.org/10.1371/journal.pone.0136690
  • Durand, J., Huchet, V., Merieau, E., Usal, C., Chesneau, M., Remy, S., … Chiffoleau, E. (2015). Regulatory B Cells with a Partial Defect in CD40 Signaling and Overexpressing Granzyme B Transfer Allograft Tolerance in Rodents. Journal of Immunology (Baltimore, Md.: 1950), 195(10), 5035‑5044. https://doi.org/10.4049/jimmunol.1500429
  • Ménoret, S., De Cian, A., Tesson, L., Remy, S., Usal, C., Boulé, J.-B., … Anegon, I. (2015). Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Scientific Reports, 5, 14410. https://doi.org/10.1038/srep14410
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Ouisse, L.-H., Brusselle, L., … Anegon, I. (2015). Transgenic animals and genetic engineering techniques. Nantes, France, 2-3 July, 2015. Transgenic Research, 24(6), 1079‑1085. https://doi.org/10.1007/s11248-015-9904-6
  • Ranchoux, B., Antigny, F., Rucker-Martin, C., Hautefort, A., Péchoux, C., Bogaard, H. J., … Perros, F. (2015). Endothelial-to-mesenchymal transition in pulmonary hypertension. Circulation, 131(11), 1006‑1018. https://doi.org/10.1161/CIRCULATIONAHA.114.008750

2014

  •    Brüggemann, M., Osborn, M. J., Ma, B., Avis, S., Anegon, I., & Buelow, R. (2014). Transgenic Animals Derived by DNA Microinjection. In S. Dübel & J. M. Reichert (Éd.), Handbook of Therapeutic Antibodies (p. 77‑88). Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA. https://doi.org/10.1002/9783527682423.ch4
  • Larcher, T., Lafoux, A., Tesson, L., Remy, S., Thepenier, V., François, V., … Huchet, C. (2014). Characterization of dystrophin deficient rats: a new model for Duchenne muscular dystrophy. PloS One, 9(10), e110371. https://doi.org/10.1371/journal.pone.0110371
  • Ménoret, S., Tesson, L., Rémy, S., Usal, C., Thépenier, V., Thinard, R., … Anegon, I. (2014). Gene targeting in rats using transcription activator-like effector nucleases. Methods (San Diego, Calif.), 69(1), 102‑107. https://doi.org/10.1016/j.ymeth.2014.02.027
  • Ponce de León, V., Mérillat, A.-M., Tesson, L., Anegón, I., & Hummler, E. (2014). Generation of TALEN-mediated GRdim knock-in rats by homologous recombination. PloS One, 9(2), e88146. https://doi.org/10.1371/journal.pone.0088146
  • Remy, S., Tesson, L., Menoret, S., Usal, C., De Cian, A., Thepenier, V., … Anegon, I. (2014). Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases. Genome Research, 24(8), 1371‑1383. https://doi.org/10.1101/gr.171538.113

2013

  • Ma, B., Osborn, M. J., Avis, S., Ouisse, L.-H., Ménoret, S., Anegon, I., … Brüggemann, M. (2013). Human antibody expression in transgenic rats: comparison of chimeric IgH loci with human VH, D and JH but bearing different rat C-gene regions. Journal of Immunological Methods, 400401, 78‑86. https://doi.org/10.1016/j.jim.2013.10.007
  • Ménoret, S., Fontanière, S., Jantz, D., Tesson, L., Thinard, R., Rémy, S., … Anegon, I. (2013). Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology, 27(2), 703‑711. https://doi.org/10.1096/fj.12-219907
  • Ménoret, S., Tesson, L., Rémy, S., Thinard, R., Usal, C., Ouisse, L.-H., … Anegon, I. (2013). Technical advances in the generation of transgenic animals and in their applications. Nantes, France, June 7th 2013. Transgenic Research, 22(5), 1065‑1072. https://doi.org/10.1007/s11248-013-9736-1
  • Osborn, M. J., Ma, B., Avis, S., Binnie, A., Dilley, J., Yang, X., … Brüggemann, M. (2013). High-affinity IgG antibodies develop naturally in Ig-knockout rats carrying germline human IgH/Igκ/Igλ loci bearing the rat CH region. Journal of Immunology (Baltimore, Md.: 1950), 190(4), 1481‑1490. https://doi.org/10.4049/jimmunol.1203041

2012

  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Iscache, A.-L., Thynard, R., … Anegon, I. (2012). Transgenesis and genome analysis, Nantes, France, June 6th 2011. Transgenic Research, 21(2), 449‑456. https://doi.org/10.1007/s11248-011-9541-7

2011

  • Iscache, A.-L., Ménoret, S., Tesson, L., Rémy, S., Usal, C., Pedros, C., … Anegon, I. (2011). Effects of BCL-2 over-expression on B cells in transgenic rats and rat hybridomas. International Immunology, 23(10), 625‑636. https://doi.org/10.1093/intimm/dxr071
  • Ménoret, S., Remy, S., Tesson, L., Usal, C., Iscache, A.-L., & Anegon, I. (2011). Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. In S. Pease & T. L. Saunders (Éd.), Advanced Protocols for Animal Transgenesis: An ISTT Manual (p. 117‑135). Berlin, Heidelberg: Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-20792-1_7
  • Tesson, L., Usal, C., Ménoret, S., Leung, E., Niles, B. J., Remy, S., … Cost, G. J. (2011). Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology, 29(8), 695‑696. https://doi.org/10.1038/nbt.1940

2010

  • Geurts, A. M., Cost, G. J., Rémy, S., Cui, X., Tesson, L., Usal, C., … Buelow, R. (2010). Generation of gene-specific mutated rats using zinc-finger nucleases. Methods in Molecular Biology (Clifton, N.J.), 597, 211‑225. https://doi.org/10.1007/978-1-60327-389-3_15
  • Ménoret, S., Iscache, A.-L., Tesson, L., Rémy, S., Usal, C., Osborn, M. J., … Anegon, I. (2010). Characterization of immunoglobulin heavy chain knockout rats. European Journal of Immunology, 40(10), 2932‑2941. https://doi.org/10.1002/eji.201040939
  • Ménoret, S., Remy, S., Usal, C., Tesson, L., & Anegon, I. (2010). Generation of transgenic rats by microinjection of short DNA fragments. Methods in Molecular Biology (Clifton, N.J.), 597, 81‑92. https://doi.org/10.1007/978-1-60327-389-3_6
  • Ménoret, S., Tesson, L., Remy, S., Usal, C., Iscache, A.-L., & Anegon, I. (2010). “Transgenesis, recent technical developments and applications” Nantes, 8th June 2009. Transgenic Research, 19(4), 711‑714. https://doi.org/10.1007/s11248-009-9340-6
  • Rémy, S., Tesson, L., Ménoret, S., Usal, C., Scharenberg, A. M., & Anegon, I. (2010). Zinc-finger nucleases: a powerful tool for genetic engineering of animals. Transgenic Research, 19(3), 363‑371. https://doi.org/10.1007/s11248-009-9323-7
  • Remy, S., Tesson, L., Usal, C., Menoret, S., Bonnamain, V., Nerriere-Daguin, V., … Anegon, I. (2010). New lines of GFP transgenic rats relevant for regenerative medicine and gene therapy. Transgenic Research, 19(5), 745‑763. https://doi.org/10.1007/s11248-009-9352-2
  • Remy, Séverine, Nguyen, T. H., Ménoret, S., Tesson, L., Usal, C., & Anegon, I. (2010). The use of lentiviral vectors to obtain transgenic rats. Methods in Molecular Biology (Clifton, N.J.), 597, 109‑125. https://doi.org/10.1007/978-1-60327-389-3_8
  • Tesson, L., Rémy, S., Ménoret, S., Usal, C., & Anegon, I. (2010). Analysis by quantitative PCR of zygosity in genetically modified organisms. Methods in Molecular Biology (Clifton, N.J.), 597, 277‑285. https://doi.org/10.1007/978-1-60327-389-3_19

2009

  • Cozzi, J., Anegon, I., Braun, V., Gross, A.-C., Merrouche, C., & Cherifi, Y. (2009). Pronuclear DNA injection for the production of transgenic rats. Methods in Molecular Biology (Clifton, N.J.), 561, 73‑88. https://doi.org/10.1007/978-1-60327-019-9_5
  • Geurts, A. M., Cost, G. J., Freyvert, Y., Zeitler, B., Miller, J. C., Choi, V. M., … Buelow, R. (2009). Knockout rats via embryo microinjection of zinc-finger nucleases. Science (New York, N.Y.), 325(5939), 433. https://doi.org/10.1126/science.1172447
  • Tomat, S., Russo, F. P., Guido, M., Kechagias, G., Masier, A., Calore, C., … Burra, P. (2009). Study of the microcirculation in hDAF transgenic rat livers xenoperfused with human blood. Xenotransplantation, 16(2), 83‑90. https://doi.org/10.1111/j.1399-3089.2009.00519.x

2005

  • Tesson, L., Cozzi, J., Ménoret, S., Rémy, S., Usal, C., Fraichard, A., & Anegon, I. (2005). Transgenic Modifications of the Rat Genome. Transgenic Research, 14(5), 531–546. https://doi.org/10.1007/s11248-005-5077-z
  • Martin, C., Plat, M., Nerriére-Daguin, V., Coulon, F., Uzbekova, S., Venturi, E., Condé, F., Hermel, J.-M., Hantraye, P., Tesson, L., Anegon, I., Melchior, B., Peschanski, M., Le Mauff, B., Boeffard, F., Sergent-Tanguy, S., Neveu, I., Naveilhan, P., Soulillou, J.-P., … Vanhove, B. (2005). Transgenic expression of CTLA4-Ig by fetal pig neurons for xenotransplantation. Transgenic Research, 14(4), 373–384.
  • Toumaniantz, G., Seze, C., Serpillon, S., Menoret, S., Tesson, L., Anégone, I., & Gauthier, C. (2005). [Vascular beta-adrenergic remodeling in rat transgenic model over-expressing endothelial beta3-adrenoceptors]. Archives Des Maladies Du Coeur Et Des Vaisseaux, 98(7–8), 836–840.
  • Toumaniantz, G., Seze, C., Serpillon, S., Menoret, S., Tesson, L., Anégone, I., & Gauthier, C. (2005). [Vascular beta-adrenergic remodeling in rat transgenic model over-expressing endothelial beta3-adrenoceptors]. Archives Des Maladies Du Coeur Et Des Vaisseaux, 98(7–8), 836–840.

2002-2003

  • Braudeau, C., Bouchet, D., Toquet, C., Tesson, L., Ménoret, S., Iyer, S., Laboisse, C., Willis, D., Jarry, A., Buelow, R., Anegon, I., & Chauveau, C. (2003). Generation of heme oxygenase-1-transgenic rats. Experimental Biology and Medicine (Maywood, N.J.), 228(5), 466–471.
  • Stockmann, H., Verbakel, C., Okkema, P., Bonthuis, F., Menoret, S., Anegon, I., Marquet, R., & IJzermans, J. (2002). No functional benefit for hDAF-transgenic rat livers despite protection from tissue damage following perfusion with human serum. Transplant International: Official Journal of the European Society for Organ Transplantation, 15(12), 595–601. https://doi.org/10.1007/s00147-002-0463-4
  • Bouchet, D., Tesson, L., Ménoret, S., Charreau, B., Mathieu, P., Yagita, H., Duisit, G., & Anegon, I. (2002). Differential sensitivity of endothelial cells of various species to apoptosis induced by gene transfer of Fas ligand: Role of FLIP levels. Molecular Medicine (Cambridge, Mass.), 8(10), 612–623.
  • Ménoret, S., Aubert, D., Tesson, L., Braudeau, C., Pichard, V., Ferry, N., & Anegon, I. (2002). lacZ transgenic rats tolerant for beta-galactosidase: Recipients for gene transfer studies using lacZ as a reporter gene. Human Gene Therapy, 13(11), 1383–1390. https://doi.org/10.1089/104303402760128603
  • Aubert, D., Ménoret, S., Chiari, E., Pichard, V., Durand, S., Tesson, L., Moullier, P., Anegon, I., & Ferry, N. (2002). Cytotoxic immune response blunts long-term transgene expression after efficient retroviral-mediated hepatic gene transfer in rat. Molecular Therapy: The Journal of the American Society of Gene Therapy, 5(4), 388–396. https://doi.org/10.1006/mthe.2002.0561
  • Tesson, L., Heslan, J.-M., Ménoret, S., & Anegon, I. (2002). Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Research, 11(1), 43–48.

1999-2001

  • Verbakel, C. A., Anegon, I., Ménoret, S., Marquet, R. L., & Ijzermans, J. N. (2001). Cellular immunity overrules the protective effect of human DAF as demonstrated in an ex vivo heart perfusion model. Transplantation Proceedings, 33(1–2), 781–782.
  • Ménoret, S., Jean, M., Tesson, L., Soulillou, J.-P., Anegon, I., & Charreau, B. (2001). Cryopreservation procedure for 1-cell and two-cell stage transgenic rat embryos. Transgenics, 3, 237–242.
  • Charreau, B., Ménoret, S., Tesson, L., Azimzadeh, A., Audet, M., Wolf, P., Marquet, R., Verbakel, C., Ijzermans, J., Cowan, P., Pearse, M., d’Apice, A., Soulillou, J. P., & Anegon, I. (1999). Protection against hyperacute xenograft rejection of transgenic rat hearts expressing human decay accelerating factor (DAF) transplanted into primates. Molecular Medicine (Cambridge, Mass.), 5(9), 617–630.
  • Menoret, S., Jean, M., Tesson, L., Soulillou, J., Anegon, I., & Charreau, B. (1999). Optimization of cryopreservation procedures for rat embryos. Transplantation Proceedings, 31(3), 1531–1532.
  • Tesson, L., Charreau, B., Ménoret, S., Gilbert, E., Soulillou, J. P., & Anegon, I. (1999). Endothelial expression of Fas ligand in transgenic rats under the temporal control of a tetracycline-inducible system. Transplantation Proceedings, 31(3), 1533–1534.

1996-1997

  • Charreau, B., Tesson, L., Menoret, S., Buscail, J., Soulillou, J. P., & Anegon, I. (1997). Production of transgenic rats for human regulators of complement activation. Transplantation Proceedings, 29(3), 1770.
  • Charreau, B., Ménoret, S., Tesson, L., Soulillou, J.-P., & Anegon, I. (1997). Generation and use of transgenic rats. Rat Genome, 3, 125–132.
  • Charreau, B., Tesson, L., Buscail, J., Soulillou, J. P., & Anegon, I. (1996). Analysis of human CD59 tissue expression directed by the CMV-IE-1 promoter in transgenic rats. Transgenic Research, 5(6), 443–450.
  • Charreau, B., Tesson, L., Soulillou, J. P., Pourcel, C., & Anegon, I. (1996). Transgenesis in rats: Technical aspects and models. Transgenic Research, 5(4), 223–234.